gingivalis in saliva and in subgingival plaque samples, showing comparable specificity to culture and real-time PCR. Conclusion: We applied the FRET technology 

In 25 subjects, one healthy, gingivitis and periodontitis site was sampled for GCF using a strip followed by subgingival plaque sampling with a curette. Bacterial assays indicated that GCF strips removed significant numbers of bacteria when placed intracrevicularly for 5 s.

Microbial analysis of subgingival plaque samples compared to that of Comparison of subgingival bacterial sampling with oral lavage for  av T Honnér — of this study was to find a sampling technique to investigate the relationship between proteins and bacteria in subgingival biofilms. Method: Three membranes  Bengtsson L, Falsen E. Predominant bacterial species in subgingival plaque in The objectives of the present thesis were to: i) in a patient sample identify  av M Sand · 2007 — Subgingival tandsten, som är lokaliserad på tandytorna under tandköttskanten, återfinns oftast i tandmellanrummen har ”Paired samples” t-test i SPSS använts. Vid hypotesprövning för Westfelt E. Rationale of mechanical plaque control. av S Edwardsson · Citerat av 3 — man herpes virus 7 from saliva samples. J Med Virol 1999 Ericson RE, Lau CN et al.

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J Med Virol 1999 Ericson RE, Lau CN et al. Bacterial diversity in human subgingival plaque. nation included assessment of visual dental plaque (VPI), bleeding on probing (BOP) such as alveolar bone height, subgingival microflora and previous dental care will be Faktorstrukturen bör dock vidare korsvalideras i ett annat sample. reduced sample consumption, low costs, and reduced power requirements of Abstract : Mechanical removal of dental plaque is regarded as an effective  Socransky, S.S., et al., Microbial complexes in subgingival plaque. neonatal mouse calvariae using a modified dissection technique giving four samples. subgingival plackets bakte- Koparal E, Eronat C, Eronat N.In vivo assessment of dental plaque pH changes in children a sample of North Carolina children.

J Periodontol Microbiologic aspects of dental plaque. De flesta av de orala arterna/fylotyperna har upptckts i subgingival biofilm [1]. of mucosal lesions and diseases, microbi-ological samples are needed, if any Holmstrup P, Schitz AW, Westergaard J. Effect of dental plaque  plaque, amyloid · plant pathology · plant dispersal subgingival skrapning · sturge-webers syndrom lot quality assurance sampling · långtidsdepression  Subgingival plaque sampling after combined mechanical and Foto.

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for example displays different bacterial profiles than plaque samples. 1 May 2018 Twenty-one subgingival plaque samples were collected from three patients with chronic periodontitis (ChP), three patients with aggressive  In this investigation, we determined whether the association between BANA hydrolysis and spirochetes could be obtained in pooled subgingival plaque samples  6 Jan 2021 Subgingival plaque samples from 587 dogs were included in the study. Breed size and age are potential risk factors for periodontitis and  In this report over 400 subgingival plaque samples taken from over 110 patients were examined microscopically and culturally for 30 bacterial parameters. Abstract.

group Sample type Sampling method Metagenomic analysis Main Findings Authors’ Conclusion 1 Pre–post interventional study 31 enrolled 25 completed SRP NA Saliva and subgingival plaque Samples collected at baseline, and 2 weeks, 6 weeks, and 12 weeks after intervention: 1. Chewing-stimulated saliva sample collected from 8 a.m. to 3 p.m. 2.

Microbial sampling was undertaken at the three deepest interproximal sites of non-adjacent teeth in every patient. Sampling in three out of four quadrants was conducted wherever possible.

Conclusion: The novel low abrasive air polishing powder is 2017-08-11 · Objectives The purpose of this study was to compare microbial profiles of saliva, pooled and site-specific subgingival samples in patients with periodontitis.
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The technique of subgingival sampling may conjecturally influence the identification and enumeration of microorganisms reported. In this study, paper point sampling and scaler sampling are compared. Methods: Samples were taken from the following intraoral sites in 35 patients with untreated chronic periodontitis before and 1.5, 3, and 6 months after non-surgical periodontal therapy: supra- and subgingival plaque from the deepest pockets in each sextant; pooled supra- and subgingival plaque from another six randomly selected, less affected teeth; mucosal swab samples from the tongue, tonsils, throat, and buccal mucosa; and stimulated and unstimulated saliva. Microbial species were A subgingival plaque sample was then obtained using a Gracey curette machined to a blade width of 0.5 mm to facilitate access to the subgingal area. The subgingival plaque from each site was collected by placing the curette at the apical extent of the gingival crevice and drawing it coronally with slight pressure against the tooth surface.

1. Oral Microbiol Immunol.
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subgingival plaque sampling strategy and analysis strategy with oligonucleotide probes Bernadette Pretzl, Jule Paul, Diana M. Krigar, Lorenz Uhlmann, Peter Eickholz & Bettina Dannewitz

Read our disclaimer for details. Of the sampling of all assessed sites, the highest probability for simultaneously detecting the tested pathogens was found in respect to the combination of supra- and subgingival plaque samples 1994-08-01 Conclusion: Salivary analysis might be discussed as a potential alternative to subgingival plaque sampling for microbiologic analysis in both AgP and CP. Discover the world's research. Subgingival plaque sampling and clinical recording (at baseline) and scaling and root planing was performed. Two weeks later the selected periodontal sites were submitted to one of the following treatments: Irrigation with a hydroalcoholic solution of propolis extract twice a week for 2 weeks (group A); irrigation with a placebo twice a week for 2 weeks (group B); or no additional treatment Before and immediately after treatment, subgingival plaque samples were taken from interdental sites with 3 to 5 mm probing depth (PD) at 2 test teeth and 2 positive control teeth. To evaluate the influence of sampling on the microflora, plaque samples were also taken twice at 2 teeth without therapy with PD of 3 to 5 mm (negative control). Collection of subgingival plaque samples with paper points is the common method for determination of periodontal pathogens.

1. Oral Microbiol Immunol. 1987 Sep;2(3):142-4. Sampling of subgingival plaque: a comparison of two methods using darkfield microscopy. Strand P, Palmer RM, Wilson RF.

However, the collection of saliva  22 Mar 2017 Material and methods: Plaque samples of 50 patients with generalized severe chronic periodontitis before therapy were pooled in two separate  Supragingival plaque should be removed with cotton pellets or periodontal scalers to secure noncontaminated subgingival specimens.

Background: The purpose of this study was to compare the results of microbiological RNA-probe analysis after subgingival plaque sampling applying two different strategies. Methods: In 220 patients, clinical examinations were obtained prior to commencement of therapy for aggressive or generalized severe chronic periodontitis (n = 113), after combined mechanical and antibiotic anti-infective Curette sampling and a subgingival washing technique were compared within and between paired sites on two separate occasions in 24 patients using darkfield microscopy. Significantly lower mean proportions of spirochetes and motile rods and higher proportions of non‐motile organisms were obtained with subgingival washing compared with curette sampling.